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Effekten av molekylvikt på hyaluronans broskgränssmörjande förmåga ensamt och i kombination med proteoglycan 4

J J Kwiecinski, S G Dorosz, T E Ludwig, S Abubacker, Mary K Cowman, T A Schmidt
HyaluronanBrosk gränssmörjningProteoglykan 4 (PRG4)
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Nyckelinsikter
  1. 01Hyaluronan med högre molekylvikt smörjer brosk mer effektivt
  2. 02Proteinet PRG4 minskar också friktionen mellan broskytor
  3. 03PRG4 och hyaluronan samverkar för att förbättra smörjningen
  4. 04Denna kombinerade effekt är oberoende av hyaluronans molekylvikt
  5. 05PRG4 kan kompensera för hyaluronan med lägre molekylvikt

Hyaluronans smörjande förmåga beror på dess molekylvikt, men proteinet PRG4 kan förbättra effekten även för hyaluronan av lägre kvalitet.

Abstract

Objectives: (1) assess the molecular weight dependence of hyaluronan's (HA) cartilage boundary lubricating ability, alone and in combination with proteoglycan 4 (PRG4), at physiological concentrations; (2) determine if HA and PRG4 interact in solution via electrophoretic mobility shift assay (EMSA).

Methods: The cartilage boundary lubricating ability of a broad range of MW HA (20 kDa, 132 kDa, 780 kDa, 1.5 MDa, and 5 MDa) at 3.33 mg/ml, both alone and in combination with PRG4 at 450 μg/ml, was assessed using a previously described cartilage-on-cartilage friction test. Static, μ(static, Neq), and kinetic, <μ(kinetic, Neq)>, were calculated. An EMSA was conducted with PRG4 and monodisperse 150 kDa and 1,000 kDa HA.

Results: Friction coefficients were reduced by HA, in a MW-dependent manner. Values of <μ(kinetic, Neq)> in 20 kDa HA, 0.098 (0.089, 0.108), were significantly greater compared to both 780 kDa, 0.080 (0.072, 0.088), and 5 MDa, 0.079 (0.070, 0.089). Linear regression showed a significant correlation between both μ(static, Neq) and <μ(kinetic, Neq)>, and log HA MW. Friction coefficients were also reduced by PRG4, and with subsequent addition of HA; however the synergistic effect was not dependent on HA MW. Values of <μ(kinetic, Neq)> in PRG4, 0.080 (0.047, 0.113), were significantly greater than values of PRG4+various MW HA (similar in value, averaging 0.040 (0.033, 0.047)). EMSA indicated that migration of 150 kDa and 1,000 kDa HA was retarded when combined with PRG4 at high PRG4:HA ratios.

Conclusions: These results suggest alterations in HA MW could significantly affect synovial fluid's cartilage boundary lubricating ability, yet this diminishment in function could be circumvented by physiological levels of PRG4 forming a complex, potentially in solution, with HA.

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APA
J J Kwiecinski, S G Dorosz, T E Ludwig, S Abubacker, Mary K Cowman, & T A Schmidt (2011). Effekten av molekylvikt på hyaluronans broskgränssmörjande förmåga ensamt och i kombination med proteoglycan 4. https://fasciaresearchdatabase.com/the-effect-of-molecular-weight-on-hyaluronans-cartilage-boundary-lubricating-ability-e-alone-and-in-combination-with-proteoglycan-4/
MLA
J J Kwiecinski, et al. "Effekten av molekylvikt på hyaluronans broskgränssmörjande förmåga ensamt och i kombination med proteoglycan 4." 2011, https://fasciaresearchdatabase.com/the-effect-of-molecular-weight-on-hyaluronans-cartilage-boundary-lubricating-ability-e-alone-and-in-combination-with-proteoglycan-4/.
Chicago
J J Kwiecinski et al. 2011. "Effekten av molekylvikt på hyaluronans broskgränssmörjande förmåga ensamt och i kombination med proteoglycan 4.". https://fasciaresearchdatabase.com/the-effect-of-molecular-weight-on-hyaluronans-cartilage-boundary-lubricating-ability-e-alone-and-in-combination-with-proteoglycan-4/