Oral Administration of 14C Labeled Gelatin Hydrolysate Leads to an Accumulation of Radioactivity in Cartilage of Mice (C57/BL), 1999
Topics: gelatin hydrolysate; peptide absorption; organ distribution; cartilage; mice (C57/BL)
Authors: Steffen Oesser, Milan Adam, Wilfried Babel and Jürgen Seifert
Several investigations showed a positive influence of orally administered gelatin on degenerative diseases of the musculo-skeletal system. Both the therapeutic mechanism and the absorption dynamics, however, remain unclear. Therefore, this study investigated the time course of gelatin hydrolysate absorption and its subsequent distribution in various tissues in mice (C57/BL). Absorption of (14)C labeled gelatin hydrolysate was compared to control mice administered (14)C labeled proline following intragastric application. Plasma and tissue radioactivity was measured over 192 h. Additional “gut sac” experiments were conducted to quantify the MW distribution of the absorbed gelatin using SDS-electrophoresis and HPLC. Ninety-five percent of enterally applied gelatin hydrolysate was absorbed within the first 12 h. The distribution of the labeled gelatin in the various tissues was similar to that of labeled proline with the exception of cartilage, where a pronounced and long-lasting accumulation of gelatin hydrolysate was observed. In cartilage, measured radioactivity was more than twice as high following gelatin administration compared to the control group. The absorption of gelatin hydrolysate in its high molecular form, with peptides of 2.5-15kD, was detected following intestinal passage. These results demonstrate intestinal absorption and cartilage tissue accumulation of gelatin hydrolysate and suggest a potential mechanism for previously observed clinical benefits of orally administered gelatin.
Related research articles
2021; The effects of collagen peptide supplementation on body composition, collagen synthesis, and recovery from joint injury and exercise: a systematic review
Topics: Fat free mass; Gelatin; Joint health; Joint pain; Muscle damage; Protein
2021; Menopause, Ultraviolet Exposure, and Low Water Intake Potentially Interact with the Genetic Variants Related to Collagen Metabolism Involved in Skin Wrinkle Risk in Middle-Aged Women
Topics: COL17A1; EGFR; MMP16; UV exposure; genetic variants; wrinkle.
2020: Recent advances in the anti-aging effects of phytoestrogens on collagen, water content, and oxidative stress
Topics: collagen; estrogen; oxidative stress; phytoestrogens; skin aging; water content
Topics: Epidemiology; Pelvic Organ Prolapse; physiopathology
Topics: exercise, jumper’s knee, loading, rehabilitation, tendon
2019: Rehabilitation and nutrition protocols for optimising return to play from traditional ACL reconstruction in elite rugby union players: A case study
Topics: ACL, collagen, rehabilitation, nutrition
2019: Protein synthesis rates of muscle, tendon, ligament, cartilage, and bone tissue in vivo in humans
Topics: Nutrition, tissue protein synthesis rate, amino acid content in tissues
2019: Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males
Topics: leucine, proline, glycine, hydroxyproline, tendon, ligament, connective tissue, athlete
2019: Intra-articular effects of combined xenogenous serum rich in growth factors (SRGF) and vitamin C on histopathology grading and staging of osteoarthritis in rat model
Topics: Osteoarthritis; SRGF; Monosodium iodoacetate; Vitamin C; Histopathology
Topics: Anterior cruciate ligament, collagen, intense physical training, volumetric changes in ligament, pre- to post season changes in ligament,
Topics: gelatin, N-terminal peptide of procollagen, exercise, musculoskeletal, injury
Topics: anatomy; pelvis; ligament; review
Topics: Collagen, triple helix
Topics: Bone mineral density; Bone turnover markers; Menopause; Osteoporosis
2017: Vitamin C–enriched gelatin supplementation before intermittent activity augments collagen synthesis
Topics: bone, exercise, ligament, tendon, inury prevention, return to play
2017: Sensitivity of ACL Volume and T2* Relaxation Time to Magnetic Resonance Imaging Scan Conditions
Topics: ACL; MRI; T2* relaxometry
Topics: training, minimizing injury, return to play, supplementation
2017; Molecular insights into prolyl and lysyl hydroxylation of fibrillar collagens in health and disease
Topics: Bruck syndrome; Collagen; Ehlers–Danlos syndrome; connective tissue disorders; fibrosis; lysyl hydroxylation; osteogenesis imperfecta; prolyl hydroxylation.
2017; Amino acid composition in determination of collagen origin and ssessment of physical factors effects
Topics: Hydroxyproline, Bone collagen, Fish skin collagen, Irradiation, Polymer
2016: Vitamin C Protects Chondrocytes against Monosodium Iodoacetate-Induced Osteoarthritis by Multiple Pathways
Topics: chondrocyte; interleukin; matrix metalloproteinase; osteoarthritis; vitamin C
2016: Stretch-induced network reconfiguration of collagen fibres in the human facet capsular ligament
Topics: collagen, fibre realignment, facet capsular
ligament, community detection,
Topics: water, collagen, osmotic pressure
Topics: ACL; exercise; tendon; ultimate tensile strength
2015: Comparative effects of vitamin C on the effects of local anesthetics ropivacaine, bupivacaine, and lidocaine on human chondrocytes
Topics: Chondrocytes; Vitamin C; Local anesthetics; Ropivacaine; Bupivacaine; Lidocaine
Topics: collagen network, mechanical adaptation, tissue remodelling
Topics: knee; articular cartilage; chondroprotection; nutraceutical
2014: Whey protein hydrolysate augments tendon and muscle hypertrophy independent of resistance exercise contraction mode
Topics: strength training, patellar tendon, eccentric training, concentric training, rate of force development, isotonic.
2014: Age- and diabetes-related nonenzymatic crosslinks in collagen fibrils: Candidate amino acids involved in Advanced Glycation End-products
Topics: Advanced glycation end-products; Ageing; Atomistic simulations; Collagen; Crosslinks; Diabetes.
Topics: collagen, advanced glycation endproducts, crosslinks, tendon mechanics, diabetes, ageing
2013: Local vitamin-C Injection Reduced Tendon Adhesion in a Chicken Model of Flexor Digitorum Profundus Tendon Injury
Topics: Vitamin C, healing tendon, adhesion,
Topics: ECM; myofibroblast; fibrosis
2012: Nutritional regulation of muscle protein synthesis with resistance exercise: strategies to enhance anabolism
Topics: Nutrition, Muscle, Anabolic intramuscular signaling, Hypertrophy
Topics: Fascial; Reinforcement; Collagen fibers; Re-orientation; Tension
2011: Optimizing an Intermittent Stretch Paradigm Using ERK1/2 Phosphorylation Results in Increased Collagen Synthesis in Engineered Ligaments
Topics: collagen synthesis, ERK 1/2 phosphorylation, intermittent stretch
2011: Change in knee osteoarthritis cartilage detected by delayed gadolinium enhanced magnetic resonance imaging following treatment with collagen hydrolysate: a pilot randomized controlled trial
Delayed gadolinium enhanced,
Magnetic resonance imaging,
2011; The presence of physiological stress shielding in the degenerative cycle of musculoskeletal disorders
Topics: Fascia; Musculoskeletal; Cerebral palsy; Remodeling; Stress shielding
Topics: Skeletal muscle; extracellular matrix; collagen; biomechanics; fibrosis
2010: Tendon and ligament fibrillar crimps give rise to left-handed helices of collagen fibrils in both planar and helical crimps
Topics: collagen handedness; fibrillar crimp; helical crimps; ligament; planar crimps; tendon
2009: Effect of Loading on the Organization of the Collagen Fibril Network in Juvenile Equine Articular Cartilage
Topics: cartilage; collagen fibril; loading; parallelism; orientation
Topics: Fascia; Tension; Contraction; Migration; Growth factor; Regulation
2007: Mechanical Stimulation Increases Collagen Type I and Collagen Type III Gene Expression of Stem Cell–Collagen Sponge Constructs for Patellar Tendon Repair
Topics: Collagen type I, collagen type III, mechanical load, patellar tendon
2007: Intra-articular injection of a nutritive mixture solution protects articular cartilage from osteoarthritic progression induced by anterior cruciate ligament transection in mature rabbits: a randomized controlled trial
Topics: Osteoarthritis, vitamin C, ascorbate, nutrition, intra articular
2007: Expression of collagen and related growth factors in rat tendon and skeletal muscle in response to specific contraction types
Topics: collagen synthesis, contraction type, concentric, eccentric, isometric
Topics: tendon metabolism; tendon mechanical function; connective tissue;
Topics: Cartilage, proteoglycans, PRG4, dynamic shear stress, static compression
Topics: articular cartilage, essential fatty acids, glucosamine sulphate, glucose transport, nutraceutical, nutrition, osteoarthritis, osteochondritis, vitamin C
2005: Myofibrillar and collagen protein synthesis in human skeletal muscle in young men after maximal shortening and lengthening contractions
Topics: eccentric; concentric; resistance exercise; z-band streaming
Topics: metabolism, matrix protein, stable-isotope tracer, proline, bone turnover, nutrition
2004: Role of Extracellular Matrix in Adaptation of Tendon and Skeletal Muscle to Mechanical Loading
Topics: ECM, collagen, tendons, skeletal muscles, mechanical loading
2003: Stress shielding of patellar tendon: effect on small-diameter collagen fibrils in a rabbit model
Topics: Stress shielding · Patellar tendon · Collagen fibril
diameter · Ultrastructure
2003: Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cultured with degraded collagen
Topics: Collagen hydrolysate; Collagen secretion;
Chondrocyte metabolism; Type II collagen; Cell culture
Topics: Exercise; Mechanotransduction; Sensitivity; Saturation; Adaptation
Topics: Early osteoarthritis, Water content, MRI, T2-mapping
2000: Effect of Mechanical Load on Articular Cartilage Collagen Structure: A Scanning Electron-Microscopic Study
Topics: Morphology; Loading, mechanical; Collagen fibers; Scanning electron microscopy; Cryofixation; Freeze
1999: Oral Administration of 14C Labeled Gelatin Hydrolysate Leads to an Accumulation of Radioactivity in Cartilage of Mice (C57/BL)
Topics: gelatin hydrolysate; peptide absorption; organ distribution; cartilage; mice (C57/BL)
1979: THE INFLUENCE OF MECHANICAL FORCES ON THE GLYCOSAMINOGLYCAN CONTENT OF THE RABBIT FLEXOR DIGITORUM PROFUNDUS TENDON
Topics: ECM, GAG, collagen, deep digital flexor tendon, loading
At the 2015 Fascia Research Congress in Washington DC, Tom Myers, the author of Anatomy Trains, gives a short introduction to Fascia and how new research changes the way we look at pain and discomfort.
Fascia eller interstitium? Fascian eller interstitium är kroppens arkitektoniska byggnadsstomme med sitt kontinuerliga flöde, som en amerikansk forskargrupp nu alltså anser vara kroppens största organ.
From Newton, to Einstein, to the new wildfire of Fascia Research. How do we understand things from a different perspective?
Fascia research has sparked a wildfire of new insights that are challenging conventional belief about how the body works – and the latest insights are presented at the 2018 Fascia Research Congress in Berlin.
Fascia is a network of connective tissue, without beginning and end, which encloses everything in the whole body, from muscles and bones, to organs and cells. But what does that mean?
What do we know about the Fascias function and what affects the Fascias ability to rebuild – and how can we use that knowledge in our everyday lives?
Exclusive interview with Gil Headly explaining how Fascia changes the perspective on how we look at the body at the 2015 Fascia Research Congress in Washington DC.
David Lesondak is a structural integrator and a myofascial specialist who has been working for many years trying to explain what fascia is, as well as the benefits you get from treating different problems with fascia treatment. In an interview at the Fascia Research Congress in Berlin 2018, he describes the basics of what fascia is and what challenges it is facing in the strive for recognition in the medical field.
One of the best ways to keep yourself healthy, free from pain and injuries is to take care of your Fascia. Here are 9 tips on how to take care of your Fascia.
Get a deeper introduction to new Fascia Research with the 2018 German documentary “The mysterious world under the skin”.