High-dose vitamin C suppresses the invasion and metastasis of breast cancer cells via inhibiting epithelial-mesenchymal transition, 2019
Topics: vitamin C, metastasis, TGF-!1, breast cancer
Authors: Ling-Hui Zeng, Qing-Mei Wang, Lin-Yi Feng, Yu-Dun Ke, Qian-Zi Xu, An-Yi Wei, Chong Zhang, Rong-Biao Ying
Purpose: Vitamin C (VC) is a kind of essential nutrient in the body regarded as a canonical antioxidant during the past hundred years. However, the anti-cancer effect of VC is controversial. Our study is trying to clarify the relationship between VC dosage and breast cancer metastasis.
Methods: Human breast cancer cell lines Bcap37 and MDA-MB-453 were treated with VC at three different concentrations (low-dose, 0.01 mM; medium-dose, 0.1 mM; high-dose, 2 mM). Wound healing assays were conducted for migration assay; transwell tests were performed to detect the ability of cell invasion. The protein levels were evaluated by Western blot analysis or immunohistochemistry. Tumor xenografts in nude mice were built to test the effects of VC on breast cancer cell proliferation and metastasis.
Results: 0.01 and 0.1 mM VC promoted cell migration and invasion when compared with the control group, but 2 mM VC significantly suppressed cell migration and invasion of breast cancer cell lines. High-dose VC increased E-cadherin and reduced Vimentin, indicating that high-dose VC suppressed epithelial-mesenchymal transition (EMT) in breast cancer cells. Besides, high-dose VC inhibited cell invasion promoted by TGF-β1 in breast cancer cells. Meanwhile, high-dose VC reversed the suppression of E-cadherin and enhancement of Vimentin induced by TGF-β1 in breast cancer cells. Furthermore, high-dose VC significantly inhibited breast cancer metastasis in vivo.
Conclusion: High-dose VC inhibits cell migration and invasion of breast cancer cell lines through suppressing EMT. Thus, it may be considered as an anticancer drug candidate for breast cancer patients.
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Frequently Asked Questions about Fascia
On the Fascia Guide FAQ we have gathered the most common questions and answers about Fascia. Is there anything you would like to know? Visit the page and submit your question.
Can you heal osteoarthritis?
The function of the articular cartilage is to reduce friction between the two articular cartilage surfaces and facilitate sliding when the joint moves, and to absorb the load when the joint is exposed to pressure, compression
What is Biotensegrity? Interview with Dr. Stephen Levin
Exclusive interview with Dr Stephen Levin, the worlds leading expert on Biotensegrity, at the Fascia Research Congress 2015.
Fascia as a flow – a new way of explaining the body’s function
By beginning to see the body as something that is alive, and not something that is dead, we have been able to begin to notice other things.
We have been preparing our whole lives to NOT be able to understand the importance of fascia
To truly understand Fascia, you must understand the whole. But how do you do that if you have trained all your life to divide and separate?
The mysterious world under the skin – German Fascia Documentary from 2018
Get a deeper introduction to new Fascia Research with the 2018 German documentary “The mysterious world under the skin”.
Training for strengthened Fascia
Exercise increases collagen production in tendons, ligaments and all Fascia. To stimulate collagen formation in tendons and ligaments, it doesn’t matter if the exercise is eccentric (muscle contraction during extension) or concentric (muscle contraction during shortening).
To understand Fascia we need to cross the bridge & see things from another perspective
Exclusive interview with Gil Headly explaining how Fascia changes the perspective on how we look at the body at the 2015 Fascia Research Congress in Washington DC.
Strolling under the skin: How does it look inside a living body?
Strolling under the skin is a fascinating journey inside a living body. With small camcorders, Dr. Jean Claeude Guimberteau has managed to capture how Fasica actually looks in a living human body.
What is fascia and in what way does fascia change the whole way of looking at the body?
Fascia is a network of connective tissue, without beginning and end, which encloses everything in the whole body, from muscles and bones, to organs and cells. But what does that mean?